Haynes:WikiTemp NBplasmids

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<!-- If you have only one plasmid, delete the |- under Plasmid 1's row, and delete the entire row for Plasmid 2.-->
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<!-- Replace Plasmid 1 and 2 with the names of your plasmids. Replace insert size and vector size with appropriate information for your plasmids. If you only have one Plasmid, delete all the text for Plasmid 2
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| &nbsp; || 15 μL --> 37°C/ 15 min.
| &nbsp; || 15 μL --> 37°C/ 15 min.
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|}
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'''DNA Sequencing Samples'''
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<!-- * signs create an automatically bulleted list. Replace 'date' with the date you submitted the samples -->
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* Submitted to DNASU on 'date'
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<!-- # signs create an automatically numbered list. Replace Plasmid 1 & 2 with the names of your plasmids. Replace 'name' with the appropriate name of each primer. -->
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# Plasmid 1 - forward primer 'name'
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# Plasmid 1 - reverse primer 'name'
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# Plasmid 2 - forward primer 'name'
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# Plasmid 2 - reverse primer 'name'

Revision as of 10:54, 3 September 2013


DNA Concentration Data


Plasmid OD260 OD260/280 ng/μL
1. Plasmid 1 --- --- ---
2. Plasmid 2 --- --- ---


Restriction Digest Table

  • Check plasmid minipreps with EcoRI/PstI digests
Reagent Volume Expected:
1. Plasmid 1 = insert size, vector size
2. Plasmid 2 = insert size, vector size
Image:GelImage.jpg
15 μL/lane; 1% agarose; Ladder
DNA(plasmid) 3.0 μL
10X buffer 1.5
EcoRI 1.0
PstI 1.0
dH2O 8.5
  15 μL --> 37°C/ 15 min.


DNA Sequencing Samples

  • Submitted to DNASU on 'date'
  1. Plasmid 1 - forward primer 'name'
  2. Plasmid 1 - reverse primer 'name'
  3. Plasmid 2 - forward primer 'name'
  4. Plasmid 2 - reverse primer 'name'
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