Haynes:WikiTemp NBplasmids: Difference between revisions
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< [http://openwetware.org/wiki/Haynes_Lab Haynes Lab OWW Home] | |||
=Plasmid Validation Template page= | =Plasmid Validation Template page= | ||
Revision as of 08:00, 3 September 2013
Plasmid Validation Template page
DNA Concentration Data
| Plasmid | OD260 | OD260/280 | ng/μL |
| 1. Plasmid 1 | --- | --- | --- |
| 2. Plasmid 2 | --- | --- | --- |
Restriction Digest Table
- Checked plasmid minipreps with EcoRI/PstI digests
| Reagent | Volume | Expected: 1. Plasmid 1 = insert size, vector size 2. Plasmid 2 = insert size, vector size |
 15 μL/lane; 1% agarose; Ladder |
| DNA(plasmid) | 3.0 μL | ||
| 10X buffer | 1.5 | ||
| EcoRI | 1.0 | ||
| PstI | 1.0 | ||
| dH2O | 8.5 | ||
| 15 μL --> 37°C/ 15 min. |
DNA Sequencing Samples
- Submitted to DNASU on 'date'
- Plasmid 1 - forward primer 'name'
- Plasmid 1 - reverse primer 'name'
- Plasmid 2 - forward primer 'name'
- Plasmid 2 - reverse primer 'name'
