Haynes:WikiTemp NBplasmids

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
(Plasmid Validation Template page)
(Plasmid Validation Template page)
Line 21: Line 21:
| Plasmid || OD260 || OD260/280 || ng/μL
| Plasmid || OD260 || OD260/280 || ng/μL
|-
|-
-
| 1. Plasmid 1 || --- || --- || ---
+
| 1. Plasmid 1 || 0.034 || 1.8 || 34.227
|-
|-
-
| 2. Plasmid 2 || --- || --- || ---
+
| 2. Plasmid 2 || 0.025 || 1.953 || 24.576
|}
|}
-
 
-
'''Restriction Digest Table'''<br>
 
-
* Checked plasmid minipreps with EcoRI/PstI digests
 
-
 
-
{| {{table}} border="1" cellspacing="3"
 
-
<!-- Editing: the coding for this table is a bit more advanced. -->
 
-
<!-- valign="top" aligns all the text in the first row to the top.  -->
 
-
<!-- The | symbols on the next two lines start new cells in the same row. This does the same thing as ||, but you have to use | on new lines to set formatting for cells. -->
 
-
<!-- bgcolor=#cfcfcf *colors* the *background* of the Reagent and Volume cells grey.  -->
 
-
<!-- The next two "rowspan=7" cells span  all 7 rows in the table so that they can fit the "Expected" list and a gel image. rowspan is how you create merged rows in Wiki code. -->
 
-
<!-- Replace Plasmid 1 and 2 with the names of your plasmids. Replace insert size and vector size with appropriate information for your plasmids. If you only have one Plasmid, delete all the text for Plasmid 2
 
-
<!-- After you upload your gel image to OWW, replace "GelImage.jpg" with the name of your image file -->
 
-
<!-- &nbsp; is ASCII code for an invisible space. -->
 
-
|- valign="top"
 
-
| bgcolor=#cfcfcf | Reagent
 
-
| bgcolor=#cfcfcf | Volume
 
-
| rowspan="7" | <u>Expected:</u><br>1. Plasmid 1 = insert size, vector size<br>2. Plasmid 2 = insert size, vector size<br>
 
-
| rowspan="7" | [[Image:GelImage.jpg|400px|Today's gel]]<br>15 μL/lane; 1% agarose; [http://openwetware.org/wiki/Image:KAH_Fermentas_GeneRuler_1kbplus.jpg Ladder]
 
-
|-
 
-
| DNA(plasmid) || 3.0 μL
 
-
|-
 
-
| 10X buffer || 1.5
 
-
|-
 
-
| EcoRI || 1.0
 
-
|-
 
-
| PstI || 1.0
 
-
|-
 
-
| dH<sub>2</sub>O || 8.5
 
-
|-
 
-
| &nbsp; || 15 μL --> 37°C/ 15 min.
 
-
|}
 
-
 
-
 
-
'''DNA Sequencing Samples'''
 
-
<!-- * signs create an automatically bulleted list. Replace 'date' with the date you submitted the samples -->
 
-
<!-- The Order Number is shown at the top of your sequencing order form. Record in the indicated spot below -->
 
-
* Submitted to DNASU on: 'date'
 
-
* Order Number:
 
-
<!-- # signs create an automatically numbered list. Replace Plasmid 1 & 2 with the names of your plasmids. Replace 'name' with the appropriate name of each primer. -->
 
-
# Plasmid 1 - forward primer 'name'
 
-
# Plasmid 1 - reverse primer 'name'
 
-
# Plasmid 2 - forward primer 'name'
 
-
# Plasmid 2 - reverse primer 'name'
 
<!--TEMPLATE CODE-->
<!--TEMPLATE CODE-->

Revision as of 15:42, 21 September 2013

< Haynes Lab OWW Home | Wiki Editing

Plasmid Validation Template page

DNA Concentration Data

Plasmid OD260 OD260/280 ng/μL
1. Plasmid 1 0.034 1.8 34.227
2. Plasmid 2 0.025 1.953 24.576


Personal tools