Haynes:WikiTemp NBplasmids

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(Plasmid Validation Template page)
(Plasmid Validation Template page)
Line 21: Line 21:
| Plasmid || OD260 || OD260/280 || ng/μL
| Plasmid || OD260 || OD260/280 || ng/μL
|-
|-
-
| 1. Plasmid 1 || 0.034 || 1.8 || 34.227
+
| 1. Plasmid 1 || --- || --- || ---
|-
|-
-
| 2. Plasmid 2 || 0.025 || 1.953 || 24.576
+
| 2. Plasmid 2 || --- || --- || ---
|}
|}
-
Yields are low, cultures will be inoculated again and miniprep will be done again
+
 
 +
 
 +
'''Restriction Digest Table'''<br>
 +
* Checked plasmid minipreps with EcoRI/PstI digests
 +
 
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{| {{table}} border="1" cellspacing="3"
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<!-- Editing: the coding for this table is a bit more advanced. -->
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<!-- valign="top" aligns all the text in the first row to the top.  -->
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<!-- The | symbols on the next two lines start new cells in the same row. This does the same thing as ||, but you have to use | on new lines to set formatting for cells. -->
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<!-- bgcolor=#cfcfcf *colors* the *background* of the Reagent and Volume cells grey.  -->
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<!-- The next two "rowspan=7" cells span  all 7 rows in the table so that they can fit the "Expected" list and a gel image. rowspan is how you create merged rows in Wiki code. -->
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<!-- Replace Plasmid 1 and 2 with the names of your plasmids. Replace insert size and vector size with appropriate information for your plasmids. If you only have one Plasmid, delete all the text for Plasmid 2
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<!-- After you upload your gel image to OWW, replace "GelImage.jpg" with the name of your image file -->
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<!-- &nbsp; is ASCII code for an invisible space. -->
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|- valign="top"
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| bgcolor=#cfcfcf | Reagent
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| bgcolor=#cfcfcf | Volume
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| rowspan="7" | <u>Expected:</u><br>1. Plasmid 1 = insert size, vector size<br>2. Plasmid 2 = insert size, vector size<br>
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| rowspan="7" | [[Image:GelImage.jpg|400px|Today's gel]]<br>15 μL/lane; 1% agarose; [http://openwetware.org/wiki/Image:KAH_Fermentas_GeneRuler_1kbplus.jpg Ladder]
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|-
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| DNA(plasmid) || 3.0 μL
 +
|-
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| 10X buffer || 1.5
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|-
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| EcoRI || 1.0
 +
|-
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| PstI || 1.0
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|-
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| dH<sub>2</sub>O || 8.5
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|-
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| &nbsp; || 15 μL --> 37°C/ 15 min.
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|}
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'''DNA Sequencing Samples'''
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<!-- * signs create an automatically bulleted list. Replace 'date' with the date you submitted the samples -->
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<!-- The Order Number is shown at the top of your sequencing order form. Record in the indicated spot below -->
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* Submitted to DNASU on: 'date'
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* Order Number:
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<!-- # signs create an automatically numbered list. Replace Plasmid 1 & 2 with the names of your plasmids. Replace 'name' with the appropriate name of each primer. -->
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# Plasmid 1 - forward primer 'name'
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# Plasmid 1 - reverse primer 'name'
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# Plasmid 2 - forward primer 'name'
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# Plasmid 2 - reverse primer 'name'
 +
 
<!--TEMPLATE CODE-->
<!--TEMPLATE CODE-->

Revision as of 17:19, 21 September 2013

< Haynes Lab OWW Home | Wiki Editing

Plasmid Validation Template page

DNA Concentration Data

Plasmid OD260 OD260/280 ng/μL
1. Plasmid 1 --- --- ---
2. Plasmid 2 --- --- ---


Restriction Digest Table

  • Checked plasmid minipreps with EcoRI/PstI digests
Reagent Volume Expected:
1. Plasmid 1 = insert size, vector size
2. Plasmid 2 = insert size, vector size
Image:GelImage.jpg
15 μL/lane; 1% agarose; Ladder
DNA(plasmid) 3.0 μL
10X buffer 1.5
EcoRI 1.0
PstI 1.0
dH2O 8.5
  15 μL --> 37°C/ 15 min.


DNA Sequencing Samples

  • Submitted to DNASU on: 'date'
  • Order Number:
  1. Plasmid 1 - forward primer 'name'
  2. Plasmid 1 - reverse primer 'name'
  3. Plasmid 2 - forward primer 'name'
  4. Plasmid 2 - reverse primer 'name'



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