Haynes Lab

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CURRENT PROJECTS<br>
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CURRENT SYNTHETIC BIOMEDICAL ENGINEERING PROJECTS<br>
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* '''Synthetic chromatin for human cell differentiation''' - Behzad Damadzadeh
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* '''Microbial communication with synthetic quorum sensing''' - Rene Davis
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'''Synthetic chromatin for cell differentiation'''
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* '''Engineering epigenetic transcription factors''' - Brady Laughlin (FURI)
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* David Barclay (FURI & SynBERC, BS) - pancreas, Jan Simper (FURI, BS) - iPSC's
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* '''Protein-DNA fusions in living microbes''' - Ryan Muller (SOLUR)
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* Description: Testing the "PcTF" synthetic chromatin protein/transcription activator described in [http://www.ncbi.nlm.nih.gov/pubmed/21669865 Haynes & Silver 2011] to determine its ability to alter the phenotypes of healthy cells, such as cell fate switching from alpha cells to beta cells in the pancreas to treat diabetes or the growing of programmable "organoids" from and transfection of iPS cells to enhance current measurements of pluripotency.
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* '''Synthetic chromatin and UV-damage''' - Brendan Fries (SOLUR)
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* '''K-12 mammalian cell synthetic biology with Open-PCR and a single drop fluorimeter''' - Alfonso Rivera-Passapera and Zazu Moloi (SynBERC)
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'''Editing synthetic genes using CRISPR'''
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* René Davis (Biological Design, PhD)
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* Description: Characterizing chromatin/CRISPR interactions. Re-engineering synthetic gene circuits in human cells using CRISPR.
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 +
 
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'''Engineering synthetic chromatin transcription factors'''
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* Cameron Gardner (FURI, BS)
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* Description: Building and testing re-engineered versions of the "PcTF" synthetic chromatin protein/ transcription activator described in [http://www.ncbi.nlm.nih.gov/pubmed/21669865 Haynes & Silver 2011].
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 +
 
 +
'''Microbial communication with synthetic quorum sensing'''
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* René Davis (Biological Design, PhD), Ryan Muller (SOLUR, BS)
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* Description: Characterizing cross-talk between decoupled cell-cell communication systems from bacteria.
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'''Lab Notebooks - Active''' {{hide|
'''Lab Notebooks - Active''' {{hide|
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* [http://openwetware.org/wiki/User:Behzad_Damadzadeh/Notebook/PcTF_Subcloning_in_E-coli Behzad]
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* [http://openwetware.org/wiki/User:David_Benjamin_Nyer/Notebook Ben]
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* [http://openwetware.org/wiki/Haynes_Lab:Notebook/Engineering_PC-TFs Brady]
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* [http://openwetware.org/wiki/Haynes_Lab:Notebook/Engineering_PC-TFs Cameron]
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* [http://openwetware.org/wiki/Haynes_Lab:Notebook/David_Barclay_Undergrad_Training/Plasmid_Transformation David Barclay]
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* [http://openwetware.org/wiki/User:David_Barclay/Notebook David Barclay]
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* [http://openwetware.org/wiki/Haynes_Lab:Notebook/Heathers_Lab_Notebook Heather]
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* [http://openwetware.org/wiki/Haynes_Lab:Notebook/Jan Jan]
* [http://openwetware.org/wiki/Haynes_Lab:Notebook/Jan Jan]
* [http://openwetware.org/wiki/User:Karmella_Haynes/Notebook Karmella]
* [http://openwetware.org/wiki/User:Karmella_Haynes/Notebook Karmella]
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* [http://openwetware.org/wiki/Haynes_Lab:Notebook/Lab_Traning_-_Pradyumna_Kadambi#Pradyumna_Kadambi.2FUndergraduate_Lab_Traning Prad]
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* [http://openwetware.org/wiki/User:Rene_M_Davis/Notebook Rene]
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* [http://openwetware.org/wiki/User:Rene_M_Davis/Notebooks René]
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* [http://openwetware.org/wiki/Haynes_Lab:Notebook:Shared/Cloning_Bootcamp SHARED: Cloning Bootcamp]
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* [http://openwetware.org/wiki/Haynes_Lab:Notebook/Short_Projects SHARED: Short Projects]
}}
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'''Lab Notebooks - Archived''' {{hide|
'''Lab Notebooks - Archived''' {{hide|
* [http://openwetware.org/wiki/Haynes_Lab:Notebook/Chimera_Biosensor_%28C.R.SYS%29 Abhi]
* [http://openwetware.org/wiki/Haynes_Lab:Notebook/Chimera_Biosensor_%28C.R.SYS%29 Abhi]
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* [http://openwetware.org/wiki/Haynes_Lab:Notebook/SynBERC_Scholars_2013 Alfonso & Zazu]
* [http://openwetware.org/wiki/Haynes_Lab:Notebook/Inteins Amanda]
* [http://openwetware.org/wiki/Haynes_Lab:Notebook/Inteins Amanda]
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* [http://openwetware.org/wiki/User:Behzad_Damadzadeh/Notebook/PcTF_Subcloning_in_E-coli Behzad]
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* [http://openwetware.org/wiki/User:Brendan_F._Fries/Notebook/Brendan_Fries Brendan]
* [http://openwetware.org/wiki/Carly's_Lab_Notebook Carly]
* [http://openwetware.org/wiki/Carly's_Lab_Notebook Carly]
* [http://openwetware.org/wiki/User:David_Dreher/Notebook/Chromatin_controlled_cell_pattern David Dreher]
* [http://openwetware.org/wiki/User:David_Dreher/Notebook/Chromatin_controlled_cell_pattern David Dreher]

Current revision

link=Haynes_Lab
link=http://engineering.asu.edu/

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Our group uses synthetic, systems, and quantitative biology to engineer useful gene and protein-based biological devices and to deepen our understanding of molecular cell biology. We operate biological devices primarily in human/ mammalian cells. Accelerating the pace of therapeutic technologies (such as tissue regeneration and customizable protein-based drugs) via modular design is the grand challenge that shapes our research plans.

CURRENT SYNTHETIC BIOMEDICAL ENGINEERING PROJECTS

Synthetic chromatin for cell differentiation

  • David Barclay (FURI & SynBERC, BS) - pancreas, Jan Simper (FURI, BS) - iPSC's
  • Description: Testing the "PcTF" synthetic chromatin protein/transcription activator described in Haynes & Silver 2011 to determine its ability to alter the phenotypes of healthy cells, such as cell fate switching from alpha cells to beta cells in the pancreas to treat diabetes or the growing of programmable "organoids" from and transfection of iPS cells to enhance current measurements of pluripotency.


Editing synthetic genes using CRISPR

  • René Davis (Biological Design, PhD)
  • Description: Characterizing chromatin/CRISPR interactions. Re-engineering synthetic gene circuits in human cells using CRISPR.


Engineering synthetic chromatin transcription factors

  • Cameron Gardner (FURI, BS)
  • Description: Building and testing re-engineered versions of the "PcTF" synthetic chromatin protein/ transcription activator described in Haynes & Silver 2011.


Microbial communication with synthetic quorum sensing

  • René Davis (Biological Design, PhD), Ryan Muller (SOLUR, BS)
  • Description: Characterizing cross-talk between decoupled cell-cell communication systems from bacteria.

Lab Notebooks - Active

Lab Notebooks - Archived

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