Haynes Lab: Difference between revisions

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CURRENT SYNTHETIC BIOMEDICAL ENGINEERING PROJECTS<br>
CURRENT SYNTHETIC BIOMEDICAL ENGINEERING PROJECTS<br>


'''Synberc: Synthetic chromatin systems for cell development'''
'''Synthetic chromatin for cancer research'''
* David Barclay (BS), Jan Simper (BS), Theodore Kyriacou (BS)
* David Nyer (Research Tech), Daniel Vargas (Biological Design PhD), Stefan Tekel (Biological Design PhD), David Tze (Biomed Eng. BS)
* Description: Designing a single-cell-level fluorescent reporter that tracks changes in epigenetic states in developing cells.
* Description: Using the "PcTF" synthetic chromatin protein/ transcription activator described in [http://www.ncbi.nlm.nih.gov/pubmed/21669865 Haynes & Silver 2011] to regulate genes in cancer cells. Building and testing re-engineered versions of PcTF.




'''Editing synthetic genes using CRISPR'''
'''Opening silenced chromatin'''
* René Davis (Biological Design, PhD)
* René Daer (Biological Design, PhD), Cassandra Barrett (Biological Design, PhD)
* Description: Characterizing chromatin/CRISPR interactions. Re-engineering synthetic gene circuits in human cells using CRISPR.
* Description: Using synthetic chromatin proteins to induce a permanently re-opened state at epigenetically silenced genes.




'''Engineering synthetic chromatin transcription factors'''
'''Synthetic chromatin systems for cell development'''
* Cameron Gardner (FURI, BS)
* David Barclay (Biomedical Eng. BS), Jan Simper (Biomedical Eng. BS), Theodore Kyriacou (Biomedical Eng. BS)
* Description: Building and testing re-engineered versions of the "PcTF" synthetic chromatin protein/ transcription activator described in [http://www.ncbi.nlm.nih.gov/pubmed/21669865 Haynes & Silver 2011].
* Description: Designing a single-cell-level fluorescent reporter that tracks changes in epigenetic states in developing cells.




'''Microbial communication with synthetic quorum sensing'''
'''Microbial communication with synthetic quorum sensing'''
* René Davis (Biological Design, PhD), Ryan Muller (SOLUR, BS)
* René Daer (Biological Design, PhD), Ryan Muller (Biochem. BS), Jiaqi Wu (Biomedical Eng. BS)
* Description: Characterizing cross-talk between decoupled cell-cell communication systems from bacteria.
* Description: Characterizing cross-talk between decoupled cell-cell communication systems from bacteria.


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'''Lab Notebooks - Active''' {{hide|
'''Lab Notebooks - Active''' {{hide|
* [http://openwetware.org/wiki/User:David_Benjamin_Nyer/Notebook Ben]
* [http://openwetware.org/wiki/User:David_Benjamin_Nyer/Notebook Ben]
* [http://openwetware.org/wiki/Haynes_Lab:Notebook/Engineering_PC-TFs Cameron]
* [http://openwetware.org/wiki/User:Daniel_A._Vargas/Notebook/General_lab_notebook Daniel]
* [http://openwetware.org/wiki/User:David_Barclay/Notebook David Barclay]
* [http://openwetware.org/wiki/User:David_Barclay/Notebook David Barclay]
* [http://openwetware.org/wiki/Haynes_Lab:Notebook/Synthetic_Chromatin_for_Cancer_Research David Tze]
* [http://openwetware.org/wiki/Haynes_Lab:Notebook/Jan Jan]
* [http://openwetware.org/wiki/Haynes_Lab:Notebook/Jan Jan]
* [http://openwetware.org/wiki/User:Karmella_Haynes/Notebook Karmella]
* [http://openwetware.org/wiki/User:Karmella_Haynes/Notebook Karmella]
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* [http://openwetware.org/wiki/User:Behzad_Damadzadeh/Notebook/PcTF_Subcloning_in_E-coli Behzad]
* [http://openwetware.org/wiki/User:Behzad_Damadzadeh/Notebook/PcTF_Subcloning_in_E-coli Behzad]
* [http://openwetware.org/wiki/User:Brendan_F._Fries/Notebook/Brendan_Fries Brendan]
* [http://openwetware.org/wiki/User:Brendan_F._Fries/Notebook/Brendan_Fries Brendan]
* [http://openwetware.org/wiki/Haynes_Lab:Notebook/Engineering_PC-TFs Cameron]
* [http://openwetware.org/wiki/Carly's_Lab_Notebook Carly]
* [http://openwetware.org/wiki/Carly's_Lab_Notebook Carly]
* [http://openwetware.org/wiki/User:David_Dreher/Notebook/Chromatin_controlled_cell_pattern David Dreher]
* [http://openwetware.org/wiki/User:David_Dreher/Notebook/Chromatin_controlled_cell_pattern David Dreher]

Revision as of 14:59, 13 August 2015


Our group uses synthetic, systems, and quantitative biology to engineer useful gene and protein-based biological devices and to deepen our understanding of molecular cell biology. We operate biological devices primarily in human/ mammalian cells. Accelerating the pace of therapeutic technologies (such as tissue regeneration and customizable protein-based drugs) via modular design is the grand challenge that shapes our research plans.

CURRENT SYNTHETIC BIOMEDICAL ENGINEERING PROJECTS

Synthetic chromatin for cancer research

  • David Nyer (Research Tech), Daniel Vargas (Biological Design PhD), Stefan Tekel (Biological Design PhD), David Tze (Biomed Eng. BS)
  • Description: Using the "PcTF" synthetic chromatin protein/ transcription activator described in Haynes & Silver 2011 to regulate genes in cancer cells. Building and testing re-engineered versions of PcTF.


Opening silenced chromatin

  • René Daer (Biological Design, PhD), Cassandra Barrett (Biological Design, PhD)
  • Description: Using synthetic chromatin proteins to induce a permanently re-opened state at epigenetically silenced genes.


Synthetic chromatin systems for cell development

  • David Barclay (Biomedical Eng. BS), Jan Simper (Biomedical Eng. BS), Theodore Kyriacou (Biomedical Eng. BS)
  • Description: Designing a single-cell-level fluorescent reporter that tracks changes in epigenetic states in developing cells.


Microbial communication with synthetic quorum sensing

  • René Daer (Biological Design, PhD), Ryan Muller (Biochem. BS), Jiaqi Wu (Biomedical Eng. BS)
  • Description: Characterizing cross-talk between decoupled cell-cell communication systems from bacteria.

Lab Notebooks - Active

Lab Notebooks - Archived

Benchling
Trello Boards

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