Haynes Lab:Notebook/ASU iGEM/2012/06/20: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
(Autocreate 2012/06/20 Entry for Haynes_Lab:Notebook/ASU_iGEM)
 
Line 6: Line 6:
| colspan="2"|
| colspan="2"|
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
==Entry title==
==6-20-12==
* Insert content here...
* Plated negative control on LB Amp plate
* Liquid cultures of T7 promoter and constitutive promoter
*Transformation (LSE)
:*Transformed DNA:
::*RBS (well 1:1H BBa_B0030)
::*TetR GFP (well 2:8A Part:BBa_I13522)
:* Cells: DH5 alpha (donated)
:* Protocol from: http://tools.invitrogen.com/content/sfs/manuals/subcloningefficiencydh5alpha_man.pdf (30 minute transformation)
:* Controls: puc19, no DNA
 





Revision as of 18:27, 3 July 2012

Project name <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

6-20-12

  • Plated negative control on LB Amp plate
  • Liquid cultures of T7 promoter and constitutive promoter
  • Transformation (LSE)
  • Transformed DNA:
  • RBS (well 1:1H BBa_B0030)
  • TetR GFP (well 2:8A Part:BBa_I13522)