Haynes Lab:Notebook/CRISPR Editing/2014/07/04: Difference between revisions
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Annealing gRNA oligos<br> | Annealing gRNA oligos<br> | ||
Revision as of 14:59, 8 July 2014
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07/04/2014Annealing gRNA oligos 3ul 100uM top oligo 20 ul reactions
Digest of pX330 and pX335. 4ug of each in 80ul reaction, 4ul BbsI. Left at 37°C for about 25 minutes. gel extracted
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