Haynes Lab:Notebook/CRISPR Editing/2014/07/06: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
(Autocreate 2014/07/06 Entry for Haynes_Lab:Notebook/CRISPR_Editing)
 
(One intermediate revision by the same user not shown)
Line 6: Line 6:
| colspan="2"|
| colspan="2"|
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
==Entry title==
==07/06/2014==
* Insert content here...
Results from yesterday's ligation <br>
{| {{table}}
| align="center" style="background:#f0f0f0;"|'''Backbone'''
| align="center" style="background:#f0f0f0;"|'''Insert'''
| align="center" style="background:#f0f0f0;"|'''# colonies'''
|-
| pX330||bb ctrl||13
|-
| pX330||g14||30
|-
| pX330||g5||20
|-
| pX330||g13||29
|-
| pX330||gn1T||21
|-
| pX330||gn1B||8
|-
| pX330||gn3T||22
|-
| pX330||gnBb B||20
|-
| pX335||bb ctrl||19
|-
| pX335||gn3T||14
|-
| pX335||gn1B||19
|-
| pX335||gn1T||23
|-
| pX335||gn3bB||23
|}
 
<br><br>
 
Zhang lab help pages say overnight ligations can lead to many colonies on the backbone plate. Picked 6 colonies from plate pX330-g14 plate.  





Revision as of 14:58, 8 July 2014

Project name <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

07/06/2014

Results from yesterday's ligation

Backbone Insert # colonies
pX330 bb ctrl 13
pX330 g14 30
pX330 g5 20
pX330 g13 29
pX330 gn1T 21
pX330 gn1B 8
pX330 gn3T 22
pX330 gnBb B 20
pX335 bb ctrl 19
pX335 gn3T 14
pX335 gn1B 19
pX335 gn1T 23
pX335 gn3bB 23



Zhang lab help pages say overnight ligations can lead to many colonies on the backbone plate. Picked 6 colonies from plate pX330-g14 plate.