Haynes Lab:Notebook/CRISPR Editing/2014/07/11

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(Autocreate 2014/07/11 Entry for Haynes_Lab:Notebook/CRISPR_Editing)
Current revision (17:34, 15 July 2014) (view source)
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==07//2014==
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==07/11/2014==
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Undergrad Cameron Gardner mini prepped cells picked from plates 5 and 14. The cells picked from plate 13 didn't grow. <br>
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Cameron cut with BbsI and EcoRI. <br>
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''Uncut and single cut plasmid should give two bands because there should be one-two BbsI bands bands 5.2kb and 3.2kb. Double cut backbone and correct ligations should give one 8kb band because there should not be a BbsI band.''
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[[Image:14.07.11 gel.png|700px|Digest gel]]
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07/11/2014

Undergrad Cameron Gardner mini prepped cells picked from plates 5 and 14. The cells picked from plate 13 didn't grow.
Cameron cut with BbsI and EcoRI.
Uncut and single cut plasmid should give two bands because there should be one-two BbsI bands bands 5.2kb and 3.2kb. Double cut backbone and correct ligations should give one 8kb band because there should not be a BbsI band.
Digest gel


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