Haynes Lab:Notebook/CRISPR Editing/2014/07/14
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07/14/2014Ligation of the other gRNAs.
gRNA 13, 1T, 1B, 3T, 3B Followed the protocol exactly. http://www.genome-engineering.org/crispr/wp-content/uploads/2014/05/CRISPR-Reagent-Description-Rev20140509.pdf Transformed 2μL of ligation reaction into 60μL of DH5αT competent cells. Long transformation, 950μL SOC.
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