Haynes Lab:Notebook/CRISPR Editing/2014/08/03: Difference between revisions
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{| {{table}} | {| {{table}} | ||
|- | |- | ||
| Reagent | (Single) | x21.5 | | Reagent || (Single) || x21.5 | ||
|- | |- | ||
| 2x Roche SYBR | 7.5 | 161.25 | | 2x Roche SYBR || 7.5 || 161.25 | ||
|- | |- | ||
| 750 nM F/R primers | 3.0 | 64.5 | | 750 nM F/R primers || 3.0 || 64.5 | ||
|- | |- | ||
| Total | 10.5 | 225.75 | | Total || 10.5 || 225.75 | ||
|} | |} | ||
Line 59: | Line 59: | ||
# MMP12 C4 | # MMP12 C4 | ||
# GAPDH A3 | # GAPDH A3 | ||
{| {{table}} | |||
|- | |||
| Reagent || (Single) || x6.5 | |||
|- | |||
| DNA (20 ng) or dH<sub>2</sub>O || up to 4.5 || --- | |||
|- | |||
| dH<sub>2</sub>O || --- || --- | |||
|- | |||
| Total || 4.5 || 29.5 | |||
|} | |||
Revision as of 10:48, 4 August 2014
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08/03/2014
Gal4EED/luc transfection
qPCR on luciferase - primer test 'Templates Master Mixes
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