Haynes Lab:Notebook/CRISPR Editing/2014/08/04: Difference between revisions

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'''Master reaction list'''
'''Master reaction list'''
{|
| style="background:#f0f0f0;"|'''Rxn #'''
| style="background:#f0f0f0;"|'''Template'''
| style="background:#f0f0f0;"|'''Target (primers)'''
| style="background:#f0f0f0;"|'''Method'''
|-
| 1||1||q2F_q3R||CNV melt
|-
| 2||1||P3F_q3R||CNV melt
|-
| 3||1||GAPDH B2||CNV melt
|-
| 4||Blank||q2F_q3R||CNV melt
|-
| 5||Blank||P3F_q3R||CNV melt
|-
| 6||Blank||GAPDH B2||CNV melt
|-
| 7||nTc||q2F_q3R||CNV melt
|-
| 8||nTc||P3F_q3R||CNV melt
|-
| 9||nTc||GAPDH B2||CNV melt
|-
| 10||4||q2R_q2F||US specific
|-
| 11||4||P3F_q3R||US specific
|-
| 12||4||GAPDH B2||US specific
|-
| 13||Blank||q2R_q2F||US specific
|-
| 14||Blank||P3F_q3R||US specific
|-
| 15||Blank||GAPDH B2||US specific
|-
| 16||nTc||q2R_q2F||US specific
|-
| 17||nTc||P3F_q3R||US specific
|-
| 18||nTc||GAPDH B2||US specific
|-
| 19||2||q1F_q1R||CNV melt
|-
| 20||2||GAPDH B2||CNV melt
|-
| 21||4||q2F_P2R||CNV melt
|-
| 22||4||GAPDH B2||CNV melt
|-
| 23||Blank||q1F_q1R||CNV melt
|-
| 24||Blank||q2F_P2R||CNV melt
|-
| 25||Blank||GAPDH B2||CNV melt
|-
| 26||nTc||q1F_q1R||CNV melt
|-
| 27||nTc||q2F_P2R||CNV melt
|-
| 28||nTc||GAPDH B2||CNV melt
|}




Revision as of 12:59, 4 August 2014

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08/04/14

  • mCherry CRISPR qPCR


mCherry CRISPR qPCR
Experimental samples:

  1. "1" - gRNA1/Cas9
  2. "2" - gRNA2/Cas9
  3. "4" - gRNA4T/Cas9n, gRNA4B/Cas9n
  • Extracted genomic DNA from these using the QIAmp genomic DNA mini kit (8/03/14)

Master reaction list

Rxn # Template Target (primers) Method
1 1 q2F_q3R CNV melt
2 1 P3F_q3R CNV melt
3 1 GAPDH B2 CNV melt
4 Blank q2F_q3R CNV melt
5 Blank P3F_q3R CNV melt
6 Blank GAPDH B2 CNV melt
7 nTc q2F_q3R CNV melt
8 nTc P3F_q3R CNV melt
9 nTc GAPDH B2 CNV melt
10 4 q2R_q2F US specific
11 4 P3F_q3R US specific
12 4 GAPDH B2 US specific
13 Blank q2R_q2F US specific
14 Blank P3F_q3R US specific
15 Blank GAPDH B2 US specific
16 nTc q2R_q2F US specific
17 nTc P3F_q3R US specific
18 nTc GAPDH B2 US specific
19 2 q1F_q1R CNV melt
20 2 GAPDH B2 CNV melt
21 4 q2F_P2R CNV melt
22 4 GAPDH B2 CNV melt
23 Blank q1F_q1R CNV melt
24 Blank q2F_P2R CNV melt
25 Blank GAPDH B2 CNV melt
26 nTc q1F_q1R CNV melt
27 nTc q2F_P2R CNV melt
28 nTc GAPDH B2 CNV melt




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