Haynes Lab:Notebook/CRISPR Editing/2014/08/04: Difference between revisions
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| 28||nTc||GAPDH B2||CNV melt | | 28||nTc||GAPDH B2||CNV melt | ||
|} | |} | ||
'''Run PCR''' | |||
* 95°C, 3 min | |||
* 45 x [95°C, 10 sec / 57°C, 10 sec / 72°C, 10 sec] | |||
* 72°C, 30 sec | |||
* Melt: 60°C -- +0.2°C/ 5 sec --> 95°C | |||
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** CRISPR treated Peak_q1F_q1R / Peak_GAPDH = | ** CRISPR treated Peak_q1F_q1R / Peak_GAPDH = | ||
** Conclusion: | ** Conclusion: | ||
** Notes: no signal from nTc, so good primer pair with | ** Notes: no signal from nTc, so good primer pair with no primer dimer signal | ||
CRISPR 4 - CNV melt | CRISPR 4 - CNV melt | ||
* Primer pair (q2F_P2R) | * Primer pair (q2F_P2R) | ||
** Untreated control Peak_q2F_P2R / Peak_GAPDH = | ** Untreated control Peak_q2F_P2R / Peak_GAPDH = | ||
** CRISPR treated Peak_q2F_P2R | ** CRISPR treated Peak_q2F_P2R / Peak_GAPDH = | ||
** Conclusion: | ** Conclusion: | ||
** Notes: no signal from nTc, so good primer pair with | ** Notes: no signal from nTc, so good primer pair with no primer dimer signal | ||
CRISPR 4 - US-specific | CRISPR 4 - US-specific | ||
* Primer pair 1 (q2R_q2F) | * Primer pair 1 (q2R_q2F) | ||
** Untreated control | ** Untreated control Cq_q2R_q2F / Peak_GAPDH = | ||
** CRISPR treated | ** CRISPR treated Peak_q2R_q2F / Peak_GAPDH = | ||
** Conclusion: | ** Conclusion: | ||
** Notes: no signal from nTc, so good primer pair with | ** Notes: no signal from nTc, so good primer pair with no primer dimer signal | ||
CRISPR 4 - US-specific | CRISPR 4 - US-specific | ||
* Primer pair 2 (P3F_q3R) | * Primer pair 2 (P3F_q3R) | ||
** Untreated control | ** Untreated control Peak_P3F_q3R / Peak_GAPDH = | ||
** CRISPR treated | ** CRISPR treated Peak_P3F_q3R| / Peak_GAPDH = | ||
** Conclusion: | ** Conclusion: | ||
** Notes: | ** Notes: ''some signal from nTc, not-so-good primer pair'' | ||
Revision as of 06:46, 5 August 2014
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08/04/14
mCherry CRISPR qPCR
Master reaction list
CRISPR 1 - CNV melt
CRISPR 1 - CNV melt
CRISPR 2 - CNV melt
CRISPR 4 - CNV melt
CRISPR 4 - US-specific
CRISPR 4 - US-specific
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