Haynes Lab:Notebook/CRISPR Editing/2014/08/05: Difference between revisions
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'''Run PCR - Bio-Rad CFX96''' | '''Run PCR - Bio-Rad CFX96''' | ||
* 95°C, 3 min | * 95°C, 3 min | ||
* '''30x''' [95°C, 10 sec / 57°C, 10 sec / 72°C, 10 sec] | * '''30x''' [95°C, 10 sec / 57°C, 10 sec / 72°C, 10 sec (measure)] | ||
* 72°C, 30 sec | * 72°C, 30 sec | ||
* Melt: 60°C -- '''+0.1°C'''/ 5 sec --> 95°C | * Melt: 60°C -- '''+0.1°C'''/ 5 sec (measure) --> 95°C | ||
Revision as of 08:25, 5 August 2014
Project name | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | ||||||||||||||||||||||||||||||||||||||||||||||||||||
08/05/14
SURVEYOR assay PCR primers [Rene, please add this info]
PCR templates
qPCR melt curve optimization - mCherry CRISPR 1
Optimization parameters
Notes:
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