Haynes Lab:Notebook/CRISPR Editing/2014/08/07: Difference between revisions
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| Target || Genomic DNA (template) || Primers || Amplicon (bp) || Expected S-Cut fragments | | Target || Genomic DNA (template) || Primers || Amplicon (bp) || Expected S-Cut fragments | ||
|- | |- | ||
| mCherry | | mCherry gRNA1 || CRISPR 1d || qPCR 1F / P3R || 329 || ~229, 100 | ||
|- | |- | ||
| mCherry | | mCherry gRNA2 || CRISPR 2d || ### / ### || ### || | ||
|- | |||
| mCherry gRNA4 || CRISPR 2d || ### / ### || ### || | |||
|- | |||
| luciferase gRNA20 || g20 || gRNA 021 US P F / gRNA 027 ES P R || 1295 || ~165, 1130 | |||
|- | |||
| luciferase gRNA29 || g29 || gRNA 021 US P F / gRNA 027 ES P R || 1295 || ~395, 900 | |||
|} | |} | ||
Revision as of 09:25, 7 August 2014
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08/07/2014
CRISPR on chromatin: transfections
Luc-mCh swap: building the donor sequence
SURVEYOR assay
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