Haynes Lab:Notebook/CRISPR Editing/2014/08/10

From OpenWetWare

< Haynes Lab:Notebook | CRISPR Editing | 2014 | 08(Difference between revisions)
Jump to: navigation, search
(Autocreate 2014/08/10 Entry for Haynes_Lab:Notebook/CRISPR_Editing)
Current revision (08:53, 10 August 2014) (view source)
(07//2014)
 
Line 6: Line 6:
| colspan="2"|
| colspan="2"|
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
-
==07//2014==
+
==08/10/2014==
-
 
+
René did PCR clean ups, decent yield. double eluted in 40ul water.
-
 
+
<br>
 +
René set up annealing of 400ng of PCR product in 15ul total volume (1.5ul of Phusion HF buffer).
 +
<br>
 +
Ash ran gel of purified PCR product
 +
<br>
 +
René set up Surveyor assay
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->

Current revision

Project name Main project page
Previous entry      Next entry

08/10/2014

René did PCR clean ups, decent yield. double eluted in 40ul water.
René set up annealing of 400ng of PCR product in 15ul total volume (1.5ul of Phusion HF buffer).
Ash ran gel of purified PCR product
René set up Surveyor assay


Personal tools