Haynes Lab:Notebook/CRISPR Editing/2014/08/26: Difference between revisions
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'''CRISPR KAH154 - flow cytometry on dox-induced cells''' | '''CRISPR KAH154 - flow cytometry on dox-induced cells''' | ||
* Harvest cells from 6-well plates, pellet in 5 mL growth medium, and resuspend in 1 mL FACS buffer | * Harvest cells from 6-well plates, pellet in 5 mL growth medium, and resuspend in 1 mL FACS buffer | ||
* | * Sample list: | ||
# | # CRISPR 1 | ||
# CRISPR 2 | |||
# CRISPR 4 | |||
# CRISPR 1d | |||
# CRISPR 2d | |||
# CRISPR 4d | |||
# KAH154 (no dox) RFP-negative | |||
# KAH154 +dox, RFP-positive | |||
Revision as of 16:39, 26 August 2014
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8/26/14
CRISPR KAH154 - flow cytometry on dox-induced cells
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