Haynes Lab:Notebook/CRISPR Editing/2014/09/10: Difference between revisions
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==09/10/2014== | ==09/10/2014== | ||
Did a test PCR for CRISPR on chromatin samples. Looks great, will start with all samples tomorrow. | |||
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Checked Luc->AmCyan PCR donor I transfected with on a gel. The correct band is there but there's other bands too so I will do the PCR again and raise the at and reduced the amount of template to try to get rid of other bands.<br> | Checked Luc->AmCyan PCR donor I transfected with on a gel. The correct band is there but there's other bands too so I will do the PCR again and raise the at and reduced the amount of template to try to get rid of other bands.<br> | ||
Revision as of 16:34, 10 September 2014
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09/10/2014Did a test PCR for CRISPR on chromatin samples. Looks great, will start with all samples tomorrow.
Ran PCR of PCR 4c 2 (PCR 4c 3), 100μL reaction, 1μL of each primer, .5μL template.
Annealing Temp 57, et 1min
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