Haynes Lab:Notebook/CRISPR Editing/2014/09/15: Difference between revisions
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Ran 6 ul on gel. | Ran 6 ul on gel. | ||
[[Image:14.09.15 SRVYR control gel.png|400px]] | |||
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Gel | Gel | ||
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::pure 1, 2a, 2b, pure 3a, pure 3b, 4a, 4b, pure 4c, 4c3 | ::pure 1, 2a, 2b, pure 3a, pure 3b, 4a, 4b, pure 4c, 4c3 | ||
[[Image:14.09.15 PCR of Luc to AmCyan gel.png|600px]] | |||
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Revision as of 15:09, 15 September 2014
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09/15/2014Purified 'gRNA 20 +dox rep 1' PCR reaction with P197/P198, SURVEYOR Ctrl C PCR reaction, and SURVEYOR Ctrl G PCR reaction. Ran binding through twice and water elution through twice. Eluted in 30ul.
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