Haynes Lab:Notebook/CRISPR Editing/2014/09/23: Difference between revisions

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(Autocreate 2014/09/23 Entry for Haynes_Lab:Notebook/CRISPR_Editing)
 
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==07//2014==
==09/23/2014==
 
Running 2ul of each PCR rxn on a gel, with 3ul of water and 1ul of 6x loading dye.
<br>
figuring out volumes of wells so I can know exactly what volume to use for each annealing reaction.
<br>
purifying and concentrating all successful PCR rxns using column. should also figure out if 50x loading dye works.





Revision as of 09:25, 23 September 2014

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09/23/2014

Running 2ul of each PCR rxn on a gel, with 3ul of water and 1ul of 6x loading dye.
figuring out volumes of wells so I can know exactly what volume to use for each annealing reaction.
purifying and concentrating all successful PCR rxns using column. should also figure out if 50x loading dye works.