Haynes Lab:Notebook/CRISPR Editing/2014/09/30: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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== | ==09/30/2014== | ||
Annealed 4 reactions each of | |||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''''' | |||
| align="center" style="background:#f0f0f0;"|'''ul for 400ng''' | |||
| align="center" style="background:#f0f0f0;"|'''ul elution solution''' | |||
| align="center" style="background:#f0f0f0;"|'''ul water''' | |||
| align="center" style="background:#f0f0f0;"|'''ul 10x annealing buffer''' | |||
|- | |||
| gRNA 20 Luc||2.86||0.14||6||1 | |||
|- | |||
| gRNA 20 +dox 1||1.96||1.04||6||1 | |||
|- | |||
| gRNA 20 +dox 2||2.23||0.77||6||1 | |||
|- | |||
| gRNA 29 Luc||2.52||0.48||6||1 | |||
|- | |||
| gRNA 29 +dox 1||2.61||0.39||6||1 | |||
|- | |||
| gRNA 29 +dox 2||2.04||0.96||6||1 | |||
|- | |||
| Luc14||3.05||-0.05||6||1 | |||
|} | |||
<br> | |||
Added two of each with 0.5ul Surveyor and 1ul enhancer, added 1.5ul of water to the other two of each, incubate at 42deg C for 45min. | |||
<br> | |||
Ran one of each on a gel | |||
Latest revision as of 00:21, 27 September 2017
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09/30/2014Annealed 4 reactions each of
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