Haynes Lab:Notebook/CRISPR Editing/2014/09/30: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
(fix raw html notebook nav)
 
(One intermediate revision by one other user not shown)
Line 2: Line 2:
|-
|-
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}}
|-
|-
| colspan="2"|
| colspan="2"|
Line 29: Line 29:
| Luc14||3.05||-0.05||6||1
| Luc14||3.05||-0.05||6||1
|}
|}
<br>
Added two of each with 0.5ul Surveyor and 1ul enhancer, added 1.5ul of water to the other two of each, incubate at 42deg C for 45min.
<br>
Ran one of each on a gel


<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->

Latest revision as of 00:21, 27 September 2017

Project name Main project page
Previous entry      Next entry

09/30/2014

Annealed 4 reactions each of

' ul for 400ng ul elution solution ul water ul 10x annealing buffer
gRNA 20 Luc 2.86 0.14 6 1
gRNA 20 +dox 1 1.96 1.04 6 1
gRNA 20 +dox 2 2.23 0.77 6 1
gRNA 29 Luc 2.52 0.48 6 1
gRNA 29 +dox 1 2.61 0.39 6 1
gRNA 29 +dox 2 2.04 0.96 6 1
Luc14 3.05 -0.05 6 1


Added two of each with 0.5ul Surveyor and 1ul enhancer, added 1.5ul of water to the other two of each, incubate at 42deg C for 45min.
Ran one of each on a gel



Retrieved from "https://openwetware.org/mediawiki/index.php?title=Haynes_Lab:Notebook/CRISPR_Editing/2014/09/30&oldid=1015476"