Haynes Lab:Notebook/CRISPR Editing/2014/10/07: Difference between revisions
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==10/07/2014== | ==10/07/2014== | ||
Ran a gel of some of the samples from the qPCR plate to check for primer dimer products<br> | Ran a gel of some of the samples from the qPCR plate to check for primer dimer products | ||
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[[Image:14.10.07 qPCR final products edited.png|700]] | [[Image:14.10.07 qPCR final products edited.png|700]] | ||
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Weird things about this gel: | |||
:Bands in my no template control lanes | |||
:Bands are fuzzy like it's not a single product | |||
:Bands look too big | |||
:Bands with the same primer set seem to be different sizes | |||
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Possibility 1: I made a mistake and added template to my no template controls <br> | |||
::Counter: The likely way I would do this would be to add a correctly diluted sample but the Cp of these wells is later than my lowest dilution on my curve | |||
Possibility 2: I'm only measuring primer dimer <br> | |||
::Counter: Why are my dilution curves so perfect? Seems the Cp is dependent on template concentration. | |||
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Sent PCR products for Luc14 gDNA Surveyor PCR products for each gRNA out for sequencing | Sent PCR products for Luc14 gDNA Surveyor PCR products for each gRNA out for sequencing | ||
Revision as of 21:17, 7 October 2014
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10/07/2014Ran a gel of some of the samples from the qPCR plate to check for primer dimer products
Possibility 2: I'm only measuring primer dimer
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