Haynes Lab:Notebook/CRISPR Editing/2014/10/22: Difference between revisions

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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}}
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==10/22/2014==
==10/22/2014==
Sequencing
{| {{table}}
{| {{table}}
| align="center" style="background:#f0f0f0;"|'''Sample'''
| align="center" style="background:#f0f0f0;"|'''Seq sample name'''
| align="center" style="background:#f0f0f0;"|'''Template'''
| align="center" style="background:#f0f0f0;"|'''Primer'''
| align="center" style="background:#f0f0f0;"|'''Primer'''
| align="center" style="background:#f0f0f0;"|''''''
| align="center" style="background:#f0f0f0;"|'''Primer name'''
| align="center" style="background:#f0f0f0;"|'''F/R'''
| align="center" style="background:#f0f0f0;"|'''F/R'''
| align="center" style="background:#f0f0f0;"|'''Distance from gRNA'''
| align="center" style="background:#f0f0f0;"|'''Distance from gRNA'''
|-
|-
| Luc14 gRNA 20 Srvyr PCR||P197||SRVYR Luc F2||F||390
|1|| Luc14 gRNA 20 Srvyr PCR||P197||SRVYR Luc F2||F||390
|-
|-
| Luc14 gRNA 20 Srvyr PCR||P160||gRNA 029 ES P R||R||238
|2|| Luc14 gRNA 20 Srvyr PCR||P160||gRNA 029 ES P R||R||238
|-
|-
| Luc14 gRNA 21 Srvyr PCR||P197||SRVYR Luc F2||F||259
|3|| Gal4-EED +dox 1 gRNA 20 Srvyr PCR||P197||SRVYR Luc F2||F||390
|-
|-
| Luc14 gRNA 22 Srvyr PCR||P197||SRVYR Luc F2||F||341
|4|| Gal4-EED +dox 2 gRNA 20 Srvyr PCR||P160||gRNA 029 ES P R||R||238
|-
|-
| Luc14 gRNA 22 Srvyr PCR||P160||gRNA 029 ES P R||R||284
|5|| Gal4-EED +dox 1 gRNA 20 Srvyr PCR||P197||SRVYR Luc F2||F||390
|-
|-
| Luc14 gRNA 27 Srvyr PCR||P169||gRNA 026 US P F||F||172
|6|| Gal4-EED +dox 2 gRNA 20 Srvyr PCR||P160||gRNA 029 ES P R||R||238
|-
|-
| Luc14 gRNA 27 Srvyr PCR||P198||SRVYR Luc R||R||247
|7|| Luc14 gRNA 21 Srvyr PCR||P197||SRVYR Luc F2||F||259
|-
|-
| Luc14 gRNA 29 Srvyr PCR||P149||gRNA 020 ES P F||F||238
|8|| Luc14 gRNA 22 Srvyr PCR||P197||SRVYR Luc F2||F||341
|-
|-
| Luc14 gRNA 29 Srvyr PCR||P176||gRNA 030 US P R||R||195
|9|| Luc14 gRNA 22 Srvyr PCR||P160||gRNA 029 ES P R||R||284
|-
|-
| Luc14 Srvyr PCR||P197||SRVYR Luc F2||F||
|10|| Luc14 gRNA 27 Srvyr PCR||P169||gRNA 026 US P F||F||172
|-
|-
| Luc14 Srvyr PCR||P160||gRNA 029 ES P R||R||
|11|| Luc14 gRNA 27 Srvyr PCR||P198||SRVYR Luc R||R||247
|-
|-
| Luc14 Srvyr PCR||P169||gRNA 026 US P F||F||
|12|| Luc14 gRNA 29 Srvyr PCR||P149||gRNA 020 ES P F||F||238
|-
|-
| Luc14 Srvyr PCR||P198||SRVYR Luc R||R||
|13|| Luc14 gRNA 29 Srvyr PCR||P176||gRNA 030 US P R||R||195
|-
|-
| Luc14 Srvyr PCR||P149||gRNA 020 ES P F||F||
|14|| Gal4-EED +dox 1 gRNA 29 Srvyr PCR||P149||gRNA 020 ES P F||F||238
|-
|-
| Luc14 Srvyr PCR||P176||gRNA 030 US P R||R||
|15|| Gal4-EED +dox 2 gRNA 29 Srvyr PCR||P176||gRNA 030 US P R||R||195
|-
|16|| Gal4-EED +dox 1 gRNA 29 Srvyr PCR||P149||gRNA 020 ES P F||F||238
|-
|17|| Gal4-EED +dox 2 gRNA 29 Srvyr PCR||P176||gRNA 030 US P R||R||195
|-
|18|| Luc14 Srvyr PCR||P197||SRVYR Luc F2||F||
|-
|19|| Luc14 Srvyr PCR||P160||gRNA 029 ES P R||R||
|-
|20|| Luc14 Srvyr PCR||P169||gRNA 026 US P F||F||
|-
|21|| Luc14 Srvyr PCR||P198||SRVYR Luc R||R||
|-
|22|| Luc14 Srvyr PCR||P149||gRNA 020 ES P F||F||
|-
|23|| Luc14 Srvyr PCR||P176||gRNA 030 US P R||R||
|}
|}


Latest revision as of 00:28, 27 September 2017

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10/22/2014

Sequencing

Seq sample name Template Primer Primer name F/R Distance from gRNA
1 Luc14 gRNA 20 Srvyr PCR P197 SRVYR Luc F2 F 390
2 Luc14 gRNA 20 Srvyr PCR P160 gRNA 029 ES P R R 238
3 Gal4-EED +dox 1 gRNA 20 Srvyr PCR P197 SRVYR Luc F2 F 390
4 Gal4-EED +dox 2 gRNA 20 Srvyr PCR P160 gRNA 029 ES P R R 238
5 Gal4-EED +dox 1 gRNA 20 Srvyr PCR P197 SRVYR Luc F2 F 390
6 Gal4-EED +dox 2 gRNA 20 Srvyr PCR P160 gRNA 029 ES P R R 238
7 Luc14 gRNA 21 Srvyr PCR P197 SRVYR Luc F2 F 259
8 Luc14 gRNA 22 Srvyr PCR P197 SRVYR Luc F2 F 341
9 Luc14 gRNA 22 Srvyr PCR P160 gRNA 029 ES P R R 284
10 Luc14 gRNA 27 Srvyr PCR P169 gRNA 026 US P F F 172
11 Luc14 gRNA 27 Srvyr PCR P198 SRVYR Luc R R 247
12 Luc14 gRNA 29 Srvyr PCR P149 gRNA 020 ES P F F 238
13 Luc14 gRNA 29 Srvyr PCR P176 gRNA 030 US P R R 195
14 Gal4-EED +dox 1 gRNA 29 Srvyr PCR P149 gRNA 020 ES P F F 238
15 Gal4-EED +dox 2 gRNA 29 Srvyr PCR P176 gRNA 030 US P R R 195
16 Gal4-EED +dox 1 gRNA 29 Srvyr PCR P149 gRNA 020 ES P F F 238
17 Gal4-EED +dox 2 gRNA 29 Srvyr PCR P176 gRNA 030 US P R R 195
18 Luc14 Srvyr PCR P197 SRVYR Luc F2 F
19 Luc14 Srvyr PCR P160 gRNA 029 ES P R R
20 Luc14 Srvyr PCR P169 gRNA 026 US P F F
21 Luc14 Srvyr PCR P198 SRVYR Luc R R
22 Luc14 Srvyr PCR P149 gRNA 020 ES P F F
23 Luc14 Srvyr PCR P176 gRNA 030 US P R R



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