Haynes Lab:Notebook/CRISPR Editing/2014/10/22

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10/22/2014

Sample Primer ' F/R Distance from gRNA
Luc14 gRNA 20 Srvyr PCR P197 SRVYR Luc F2 F 390
Luc14 gRNA 20 Srvyr PCR P160 gRNA 029 ES P R R 238
Gal4-EED +dox 1 gRNA 20 Srvyr PCR P197 SRVYR Luc F2 F 390
Gal4-EED +dox 2 gRNA 20 Srvyr PCR P160 gRNA 029 ES P R R 238
Gal4-EED +dox 1 gRNA 20 Srvyr PCR P197 SRVYR Luc F2 F 390
Gal4-EED +dox 2 gRNA 20 Srvyr PCR P160 gRNA 029 ES P R R 238
Luc14 gRNA 21 Srvyr PCR P197 SRVYR Luc F2 F 259
Luc14 gRNA 22 Srvyr PCR P197 SRVYR Luc F2 F 341
Luc14 gRNA 22 Srvyr PCR P160 gRNA 029 ES P R R 284
Luc14 gRNA 27 Srvyr PCR P169 gRNA 026 US P F F 172
Luc14 gRNA 27 Srvyr PCR P198 SRVYR Luc R R 247
Luc14 gRNA 29 Srvyr PCR P149 gRNA 020 ES P F F 238
Luc14 gRNA 29 Srvyr PCR P176 gRNA 030 US P R R 195
Gal4-EED +dox 1 gRNA 29 Srvyr PCR P149 gRNA 020 ES P F F 238
Gal4-EED +dox 2 gRNA 29 Srvyr PCR P176 gRNA 030 US P R R 195
Gal4-EED +dox 1 gRNA 29 Srvyr PCR P149 gRNA 020 ES P F F 238
Gal4-EED +dox 2 gRNA 29 Srvyr PCR P176 gRNA 030 US P R R 195
Luc14 Srvyr PCR P197 SRVYR Luc F2 F
Luc14 Srvyr PCR P160 gRNA 029 ES P R R
Luc14 Srvyr PCR P169 gRNA 026 US P F F
Luc14 Srvyr PCR P198 SRVYR Luc R R
Luc14 Srvyr PCR P149 gRNA 020 ES P F F
Luc14 Srvyr PCR P176 gRNA 030 US P R R



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