Haynes Lab:Notebook/CRISPR Editing/2014/12/06: Difference between revisions
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'''72 hours post transfection'''<br> | '''72 hours post transfection'''<br> | ||
Harvesting the cells (following Zhang lab recommendation for HEK 293 cells. <br> | Harvesting the cells (following Zhang lab recommendation for HEK 293 cells. <br> | ||
QIAamp genomic DNA extraction kit. | |||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''Sample Read#''' | |||
| align="center" style="background:#f0f0f0;"|'''260''' | |||
| align="center" style="background:#f0f0f0;"|'''280''' | |||
| align="center" style="background:#f0f0f0;"|'''260/280''' | |||
| align="center" style="background:#f0f0f0;"|'''ng/µL''' | |||
|- | |||
| g023||0.152||0.077||1.975||152.302 | |||
|- | |||
| g025||0.146||0.073||1.991||146.123 | |||
|- | |||
| g029||0.123||0.062||1.974||122.575 | |||
|- | |||
| g030||0.111||0.056||1.998||111.309 | |||
|- | |||
| g031||0.124||0.063||1.972||124.43 | |||
|- | |||
| g032||0.136||0.069||1.983||136.408 | |||
|- | |||
| g033||0.124||0.063||1.977||123.728 | |||
|- | |||
| g034||0.139||0.071||1.968||138.986 | |||
|- | |||
| g035||0.111||0.055||2.008||110.973 | |||
|- | |||
| g036||0.125||0.064||1.971||125.365 | |||
|- | |||
| g038||0.108||0.055||1.974||108.185 | |||
|- | |||
| g039||0.09||0.047||1.928||90.499 | |||
|- | |||
| g040||0.153||0.077||1.986||153.055 | |||
|- | |||
| g041||0.127||0.065||1.961||127.455 | |||
|- | |||
| g042||0.14||0.071||1.991||140.384 | |||
|- | |||
| g043||0.126||0.064||1.962||125.746 | |||
|- | |||
| g044||0.138||0.071||1.956||138.15 | |||
|- | |||
| g045||0.151||0.077||1.97||150.75 | |||
|- | |||
| g046||0.134||0.069||1.928||133.792 | |||
|- | |||
| g048||0.13||0.066||1.965||130.029 | |||
|- | |||
| g049||0.094||0.048||1.95||94.331 | |||
|- | |||
| Untreated||0.111||0.056||1.998||110.915 | |||
|} | |||
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Revision as of 17:19, 6 December 2014
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12/06/201472 hours post transfection
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