Haynes Lab:Notebook/CRISPR Editing/2014/12/18: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
Line 29: Line 29:
| untreated||0.023||0.013||1.839||23.346
| untreated||0.023||0.013||1.839||23.346
|} all PCR for all gRNA treated cells, 2-50ul reactions gotaq at 61deg at.
|} all PCR for all gRNA treated cells, 2-50ul reactions gotaq at 61deg at.
 
Ran on gel<br>
[[image:14.12.18_gel_of_many_PCRs.png‎|600]]
<br>
Extra bands on 31, 35, and 38. Will redo PCR at higher AT


<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->

Revision as of 08:27, 19 December 2014

Project name <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

12/18/2014

Ran PCR from yesterday on gel.
PCR purified g029 PCR reactions. Yield about 30ng/ul. PCR purified PCR reactions from yesterday. Some were as low as 23ng/ul. Will redo

Sample Read# 260 280 260/280 ng/µL
g029 0.032 0.016 2.007 31.813
g029 0.029 0.015 1.899 29.381
g023 0.033 0.02 1.674 33.467
g025 0.023 0.012 1.939 23.109
g030 0.023 0.012 1.862 22.585
untreated 0.023 0.013 1.839 23.346
all PCR for all gRNA treated cells, 2-50ul reactions gotaq at 61deg at.

Ran on gel
600
Extra bands on 31, 35, and 38. Will redo PCR at higher AT


Retrieved from "https://openwetware.org/mediawiki/index.php?title=Haynes_Lab:Notebook/CRISPR_Editing/2014/12/18&oldid=848542"