Haynes Lab:Notebook/CRISPR Editing/2015/01/09: Difference between revisions
Rene M Davis (talk | contribs) |
Rene M Davis (talk | contribs) |
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Added 3.74ul 6x loading dye to the remaining 17.7ul of surveyor reaction. Ran on a large 2% agarose gel. Made the gel very thick so I could fit all the reaction in the well but some still spilled out. I also took a long time to load the gel so I think that messed up some of the early lanes. It looks like there are two bands in those lanes, even in the ladder. I think maybe I ran it too fast (100V). I need to learn more about 2% gels. The bands are consistent with the bioanalyzer data but it's not close to publishable data. | Added 3.74ul 6x loading dye to the remaining 17.7ul of surveyor reaction. Ran on a large 2% agarose gel. Made the gel very thick so I could fit all the reaction in the well but some still spilled out. I also took a long time to load the gel so I think that messed up some of the early lanes. It looks like there are two bands in those lanes, even in the ladder. I think maybe I ran it too fast (100V). I need to learn more about 2% gels. The bands are consistent with the bioanalyzer data but it's not close to publishable data. | ||
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Thawed Luc14 gal4-eed cells for experiments | |||
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Revision as of 15:44, 14 January 2015
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01/09/2015
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