Haynes Lab:Notebook/CRISPR Editing/2015/01/14: Difference between revisions
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Picked colonies from 6 of the transformation plates from yesterday into 2-6mL cultures to miniprep tomorrow. | Picked colonies from 6 of the transformation plates from yesterday into 2-6mL cultures to miniprep tomorrow. | ||
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''Testing PCR primers''<br> | '''Testing PCR primers'''<br> | ||
I have a ton of primers that amplify across my target sequence. I tested all of them by pairing all the forward primers with P198 and all the reverse primers with P197, both of which I know work very well. | I have a ton of primers that amplify across my target sequence. I tested all of them by pairing all the forward primers with P198 and all the reverse primers with P197, both of which I know work very well. | ||
:95 for 3min | :95 for 3min | ||
Revision as of 11:03, 17 January 2015
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01/14/2015Picked colonies from 6 of the transformation plates from yesterday into 2-6mL cultures to miniprep tomorrow.
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