Haynes Lab:Notebook/CRISPR Editing/2015/01/28: Difference between revisions

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Isolated gDNA from samples
{| {{table}}
| align="center" style="background:#f0f0f0;"|'''Cell type'''
| align="center" style="background:#f0f0f0;"|'''treatment'''
| align="center" style="background:#f0f0f0;"|'''rep'''
| align="center" style="background:#f0f0f0;"|'''260'''
| align="center" style="background:#f0f0f0;"|'''280'''
| align="center" style="background:#f0f0f0;"|'''260/280'''
| align="center" style="background:#f0f0f0;"|'''ng/µL'''
|-
| Luc14||g023||1||0.082||0.041||2.01||82.358
|-
| Luc14||g023||2||0.076||0.037||2.074||76.437
|-
| Luc14||g023||3||0.119||0.06||2.002||119.281
|-
| GAL4-EED +dox||g023||1||0.123||0.06||2.046||122.844
|-
| GAL4-EED +dox||g023||2||0.102||0.05||2.029||102.434
|-
| GAL4-EED +dox||g023||3||0.102||0.051||2.017||101.862
|-
| Luc14||g034||1||0.072||0.034||2.138||71.774
|-
| Luc14||g034||2||0.118||0.059||2.004||117.513
|-
| Luc14||g034||3||0.108||0.053||2.037||108.009
|-
| GAL4-EED +dox||g034||1||0.086||0.043||2||86.013
|-
| GAL4-EED +dox||g034||2||0.105||0.052||2.03||105.127
|-
| GAL4-EED +dox||g034||3||0.122||0.062||1.988||122.48
|-
| Luc14||g048||1||0.173||0.086||2.021||172.854
|-
| Luc14||g048||2||0.218||0.108||2.008||217.502
|-
| Luc14||g048||3||0.19||0.094||2.02||190.132
|-
| GAL4-EED +dox||g048||1||0.184||0.092||2.002||183.712
|-
| GAL4-EED +dox||g048||2||0.19||0.095||2.007||189.828
|-
| GAL4-EED +dox||g048||3||0.209||0.105||1.99||209.097
|-
| Luc14||Untreated||1||0.204||0.103||1.978||203.767
|-
| Luc14||Untreated||2||0.201||0.1||2.011||201.304
|-
| Luc14||Untreated||3||0.222||0.112||1.986||221.905
|-
| GAL4-EED +dox||Untreated||1||0.206||0.102||2.023||205.606
|-
| GAL4-EED +dox||Untreated||2||0.22||0.107||2.063||219.954
|-
| GAL4-EED +dox||Untreated||3||0.206||0.102||2.011||205.907
|}


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Revision as of 16:43, 28 January 2015

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01/28/2015

Mini-prepped KAH126 starting from a 2mL culture.

Sample Read# 260 280 260/280 ng/µL
KAH 126 0.453 0.237 1.913 452.756
KAH126 0.408 0.214 1.907 408.086


Isolated gDNA from samples

Cell type treatment rep 260 280 260/280 ng/µL
Luc14 g023 1 0.082 0.041 2.01 82.358
Luc14 g023 2 0.076 0.037 2.074 76.437
Luc14 g023 3 0.119 0.06 2.002 119.281
GAL4-EED +dox g023 1 0.123 0.06 2.046 122.844
GAL4-EED +dox g023 2 0.102 0.05 2.029 102.434
GAL4-EED +dox g023 3 0.102 0.051 2.017 101.862
Luc14 g034 1 0.072 0.034 2.138 71.774
Luc14 g034 2 0.118 0.059 2.004 117.513
Luc14 g034 3 0.108 0.053 2.037 108.009
GAL4-EED +dox g034 1 0.086 0.043 2 86.013
GAL4-EED +dox g034 2 0.105 0.052 2.03 105.127
GAL4-EED +dox g034 3 0.122 0.062 1.988 122.48
Luc14 g048 1 0.173 0.086 2.021 172.854
Luc14 g048 2 0.218 0.108 2.008 217.502
Luc14 g048 3 0.19 0.094 2.02 190.132
GAL4-EED +dox g048 1 0.184 0.092 2.002 183.712
GAL4-EED +dox g048 2 0.19 0.095 2.007 189.828
GAL4-EED +dox g048 3 0.209 0.105 1.99 209.097
Luc14 Untreated 1 0.204 0.103 1.978 203.767
Luc14 Untreated 2 0.201 0.1 2.011 201.304
Luc14 Untreated 3 0.222 0.112 1.986 221.905
GAL4-EED +dox Untreated 1 0.206 0.102 2.023 205.606
GAL4-EED +dox Untreated 2 0.22 0.107 2.063 219.954
GAL4-EED +dox Untreated 3 0.206 0.102 2.011 205.907


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