Haynes Lab:Notebook/CRISPR Editing/2015/01/29: Difference between revisions
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Incubated for 30 minutes at room temp.<br> | |||
::Notes from the manual: | ::Notes from the manual: | ||
:::Note: The red or yellow color of the TOPO® vector solution is normal and is used to visualize the solution. | :::Note: The red or yellow color of the TOPO® vector solution is normal and is used to visualize the solution. | ||
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*Transform | *Transform | ||
:warmed LB plates for 30 40 minutes | |||
:added 2ul and 4ul of each of the four reactions to 50ul of cells | |||
:incubate on ice for 10 minutes | |||
:plate | |||
:grow overnight at 37 in wang lab incubator | |||
Revision as of 16:42, 29 January 2015
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01/29/2015Topo cloning Added dATPs to the ends of untreated and g034 treated Luc14 P197/198 PCR products
Incubated at 72°C for 15 min in PCR machine
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