Haynes Lab:Notebook/CRISPR Editing/2015/01/30: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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Prepped KAH126 plasmids for sequencing from the mini preps we did on January 28, 2015 (Wed). | Prepped KAH126 plasmids for sequencing from the mini preps we did on January 28, 2015 (Wed). | ||
We each prepped two tubes. Each contained 7μL of water with 2μL of plasmid template and 1μL of either DD122 primer or DD123 primer. The primers were obtained from Cameron. | We each prepped two tubes. Each contained 7μL of water with 2μL of plasmid template and 1μL of either DD122 primer or DD123 primer. The primers were obtained from Cameron. | ||
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Picked 6 colonies from one of the g034 topo plates into 2mL LB+amp. Let grow overnight. | |||
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Added new media to Cameron's cells. Used media+puro from a long time ago, not sure if the puro is still good. | |||
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Did a test PCR reaction with Untreated Luc14 gDNA and phusion. Only 30 cycles and 6 different annealing temps: 66, 67, 68, 69, 70, 72. Want to see if I can reduce background bands but still get enough product. Put at -20. | |||
Latest revision as of 00:42, 27 September 2017
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01/30/2015
Prepped KAH126 plasmids for sequencing from the mini preps we did on January 28, 2015 (Wed).
We each prepped two tubes. Each contained 7μL of water with 2μL of plasmid template and 1μL of either DD122 primer or DD123 primer. The primers were obtained from Cameron.
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