Haynes Lab:Notebook/CRISPR Editing/2015/01/31: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
Rene M Davis (talk | contribs) (Autocreate 2015/01/31 Entry for Haynes_Lab:Notebook/CRISPR_Editing) |
(fix raw html notebook nav) |
||
(3 intermediate revisions by one other user not shown) | |||
Line 2: | Line 2: | ||
|- | |- | ||
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
|- | |- | ||
| colspan="2"| | | colspan="2"| | ||
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --> | <!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --> | ||
==01//2015== | ==01/30/2015== | ||
miniprepped colonies from topo cloning plate for g034 | |||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''Sample Read#''' | |||
| align="center" style="background:#f0f0f0;"|'''260''' | |||
| align="center" style="background:#f0f0f0;"|'''280''' | |||
| align="center" style="background:#f0f0f0;"|'''260/280''' | |||
| align="center" style="background:#f0f0f0;"|'''ng/µL''' | |||
|- | |||
| Topo clone 1||0.02||0.011||1.827||19.983 | |||
|- | |||
| Topo clone 2||0.057||0.03||1.882||57.143 | |||
|- | |||
| Topo clone 3||0.582||0.302||1.924||581.887 | |||
|- | |||
| Topo clone 4||0.018||0.008||2.333||17.68 | |||
|- | |||
| Topo clone 5||0.046||0.025||1.851||46.218 | |||
|- | |||
| Topo clone 6||0.04||0.021||1.896||40.046 | |||
|} | |||
<br> | |||
Ran gradient PCR reactions from yesterday on gel. Setting AT of 66 as 100%, estimated the drop in efficiency. Def not quantitative. There was an obvious off target band around 800bp that faded and disappeared at higher annealing temperatures. | |||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''Annealing Temps''' | |||
| align="center" style="background:#f0f0f0;"|'''Efficiency''' | |||
| align="center" style="background:#f0f0f0;"|'''off target band ~800bp''' | |||
|- | |||
| 66||100||yes | |||
|- | |||
| 67||100||yes | |||
|- | |||
| 68||100||yes | |||
|- | |||
| 69||80||faint to gone | |||
|- | |||
| 70||80||gone | |||
|- | |||
| 72||60||gone | |||
|} | |||
<br> | |||
Split Cameron's cells into two T-25 flasks. | |||
Latest revision as of 00:42, 27 September 2017
Project name | Main project page Previous entry Next entry | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||
01/30/2015miniprepped colonies from topo cloning plate for g034
|