Haynes Lab:Notebook/CRISPR Editing/2015/02/03: Difference between revisions
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Rene M Davis (talk | contribs) |
Rene M Davis (talk | contribs) |
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==02/03/2015== | ==02/03/2015== | ||
'''JW insert what you did here''' | '''JW insert what you did here''' | ||
Restriction digest | |||
{| {{table}} | {| {{table}} | ||
| align="center" style="background:#f0f0f0;"|'''Thing''' | | align="center" style="background:#f0f0f0;"|'''Thing''' | ||
Line 25: | Line 25: | ||
| Total||20 | | Total||20 | ||
|} | |} | ||
<br> | |||
Purified with PCR clean up kit | |||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''Sample Read#''' | |||
| align="center" style="background:#f0f0f0;"|'''260''' | |||
| align="center" style="background:#f0f0f0;"|'''280''' | |||
| align="center" style="background:#f0f0f0;"|'''260/280''' | |||
| align="center" style="background:#f0f0f0;"|'''ng/µL''' | |||
|- | |||
| insert for ryan||0.012||0.007||1.62||12.1 | |||
|} | |||
<br><br> | <br><br> | ||
Digested 400ng of purified PCR products from yesterday. Also duplicated with no enzyme, added 1ul elution solution: | Digested 400ng of purified PCR products from yesterday. Also duplicated with no enzyme, added 1ul elution solution: |
Revision as of 12:46, 3 February 2015
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02/03/2015JW insert what you did here Restriction digest
Let reaction go for 3 hours, diluted 1:10, ran on bioanalyzer
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