Haynes Lab:Notebook/CRISPR Editing/2015/02/04: Difference between revisions

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(Autocreate 2015/02/04 Entry for Haynes_Lab:Notebook/CRISPR_Editing)
 
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==02//2015==
==02/04/2015==
 
JW: analyzed sequencing results for KAH126. Sequencing failed for both minipreps for both primers. <br>Possible reasons
 
:Primers don't match the backbone
::Primers are not what we think they are
::backbone isn't what we think it is
:Miniprepped colonies are just genomic DNA
:Sequencing run failed


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Revision as of 13:55, 6 February 2015

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02/04/2015

JW: analyzed sequencing results for KAH126. Sequencing failed for both minipreps for both primers.
Possible reasons

Primers don't match the backbone
Primers are not what we think they are
backbone isn't what we think it is
Miniprepped colonies are just genomic DNA
Sequencing run failed