Haynes Lab:Notebook/CRISPR Editing/2015/02/18: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
Line 7: Line 7:
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
==02/18/2015==
==02/18/2015==
Ran test end-point PCR to determine if the qPCR primer sets I ordered to target pX330 will work for estimating transfection efficiency
Ran test end-point PCR to determine if the qPCR primer sets I ordered to target pX330 will work for estimating transfection efficiency. Used gDNA from Luc14 treated with g023 rep 1 and g023 in pX330 as templates:


{| {{table}}
{| {{table}}

Revision as of 11:07, 22 February 2015

Project name <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

02/18/2015

Ran test end-point PCR to determine if the qPCR primer sets I ordered to target pX330 will work for estimating transfection efficiency. Used gDNA from Luc14 treated with g023 rep 1 and g023 in pX330 as templates:

' For Rev
Primer set 1 P207 P208
Primer set 2 P209 P210
Primer set 3 P211 P212