Haynes Lab:Notebook/CRISPR Editing/2015/03/03: Difference between revisions
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Ran PCR products from P151/158 on gel. Only single product for each band. | Ran PCR products from P151/158 on gel. Only single product for each band. | ||
<br><br> | <br><br> | ||
Sequencing:<br>Need to purify. Will purify 25ul and elute into | Sequencing:<br>Need to purify. Will purify 25ul and elute into 35 ul. | ||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''Cell type''' | |||
| align="center" style="background:#f0f0f0;"|'''treatment''' | |||
| align="center" style="background:#f0f0f0;"|'''rep''' | |||
| align="center" style="background:#f0f0f0;"|'''260''' | |||
| align="center" style="background:#f0f0f0;"|'''280''' | |||
| align="center" style="background:#f0f0f0;"|'''260/280''' | |||
| align="center" style="background:#f0f0f0;"|'''ng/µL''' | |||
|- | |||
| Luc14||Untreated||1||0.035||0.017||2.006||35.007 | |||
|- | |||
| Luc14||g046||1||0.032||0.016||2.02||31.691 | |||
|- | |||
| Luc14||Untreated||2||0.045||0.022||2.034||44.797 | |||
|- | |||
| Luc14||Untreated||3||0.046||0.024||1.938||46.277 | |||
|- | |||
| Gal4-EED +dox||Untreated||1||0.04||0.019||2.099||40.212 | |||
|- | |||
| Gal4-EED +dox||Untreated||2||0.043||0.023||1.885||42.773 | |||
|- | |||
| Gal4-EED +dox||Untreated||3||0.044||0.023||1.93||43.675 | |||
|- | |||
| Luc14||g046||2||0.03||0.015||2.086||30.259 | |||
|- | |||
| Luc14||g046||3||0.031||0.015||2.113||31.368 | |||
|- | |||
| Gal4-EED +dox||g046||1||0.035||0.017||2.042||35.122 | |||
|- | |||
| Gal4-EED +dox||g046||2||0.039||0.019||2.05||38.501 | |||
|- | |||
| Gal4-EED +dox||g046||3||0.041||0.022||1.897||41.336 | |||
|- | |||
| Luc14||Untreated||1||0.032||0.016||1.949||31.937 | |||
|- | |||
| Luc14||g046||1||0.03||0.015||1.94||29.645 | |||
|} | |||
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Revision as of 15:05, 3 March 2015
Project name | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
02//2015Checked concentrations on minipreps from last year of Lonza pMax GFP from Krishna. very low so JW transformed the original along with pMax GFP 6.2 and KAH60.
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