Haynes Lab:Notebook/CRISPR Editing/2015/03/04: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
Line 53: Line 53:
|}
|}


 
<br><br>
Diluted untreated and g046 column purified P151/158 PCR products down to 30ng/ul each.<br>
Cut with HindIII, 20 minutes at 37deg C and 20 min at 80deg C to inactivate. Did in new PCR machine. <br>
{| {{table}}
| align="center" style="background:#f0f0f0;"|'''DNA'''
| align="center" style="background:#f0f0f0;"|'''250ng'''
|-
| enzyme||0.5
|-
| 10xbuffer||2
|-
| water||9.17
|-
| Total||20
|}
<br>
Dilute 1ul into 20ul water. run 1ul on bioanalyzer.
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->
|}
|}


__NOTOC__
__NOTOC__

Revision as of 13:41, 4 March 2015

Project name <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

03/04/2015

Cell type treatment rep 260 280 260/280 ng/µL
Luc14 g023 1 0.089 0.048 1.862 89.331
Luc14 g023 2 0.087 0.046 1.909 87.359
Luc14 g023 3 0.088 0.048 1.834 88.229
Gal4-EED +dox g023 1 0.091 0.049 1.869 91.083
Gal4-EED +dox g023 2 0.091 0.05 1.833 91.397
Gal4-EED +dox g023 3 0.096 0.052 1.832 95.767
Luc14 g045 1 0.078 0.043 1.808 77.703
Luc14 g045 2 0.029 0.016 1.75 28.731
Luc14 g045 3 0.067 0.036 1.861 66.736
Gal4-EED +dox g045 1 0.085 0.046 1.836 85.215
Gal4-EED +dox g045 2 0.073 0.041 1.788 73.213
Gal4-EED +dox g045 3 0.067 0.037 1.805 67.248
Luc14 g048 1 0.056 0.03 1.849 56.099
Luc14 g048 2 0.056 0.031 1.795 56.201
Luc14 g048 3 0.06 0.034 1.782 59.902
Gal4-EED +dox g048 1 0.076 0.041 1.844 76.457
Gal4-EED +dox g048 2 0.072 0.039 1.854 71.611
Gal4-EED +dox g048 3 0.077 0.042 1.838 77.038



Diluted untreated and g046 column purified P151/158 PCR products down to 30ng/ul each.
Cut with HindIII, 20 minutes at 37deg C and 20 min at 80deg C to inactivate. Did in new PCR machine.

DNA 250ng
enzyme 0.5
10xbuffer 2
water 9.17
Total 20


Dilute 1ul into 20ul water. run 1ul on bioanalyzer.


Retrieved from "https://openwetware.org/mediawiki/index.php?title=Haynes_Lab:Notebook/CRISPR_Editing/2015/03/04&oldid=870499"