Haynes Lab:Notebook/CRISPR Editing/2015/03/12: Difference between revisions
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Cut 250ng of g031 treated luc14 and gal4eed+dox P151/158 nested PCR products. one hour at 30degC then heat inactivated at 65 for 20 minutes. No enzyme control has 13ul water, 0ul enzyme: | |||
{| {{table}} | |||
| water||12.5 | |||
|- | |||
| DNA||5 | |||
|- | |||
| Buffer ||2 | |||
|- | |||
| Bsa1I||0.5 | |||
|- | |||
| total||20 | |||
|} | |||
Ran on BA, saw lots of uncut amplicons, equal for Luc14 and Gal4, think the enzyme didn't cut completely. | |||
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Revision as of 11:39, 13 March 2015
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03/12/2015
Ran on BA, saw lots of uncut amplicons, equal for Luc14 and Gal4, think the enzyme didn't cut completely. |