Haynes Lab:Notebook/CRISPR Editing/2015/03/20: Difference between revisions
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==03//2015== | ==03/20/2015== | ||
PCR of g025, g032, and g044 Luc14 and Gal4-EED+dox treated cells with Phusion and P197/198. | |||
<br><br> | |||
Nested PCR of g032 with P149/P174 | |||
:98 deg for 30 | |||
:30 cycles | |||
::98 for 10s | |||
::68 for 10s | |||
::72 for 15s | |||
:72 for 5 min | |||
:4 forever | |||
<br> | |||
Plated cells for transfections tomorrow. Gal4-EED+dox cells plated at 0.16x10^6 cells/well in 12 well plates. Luc14 cells plated at 0.1x10^6 cells / well in 12 well plates. Two plates for each cell type for a total of 4 12-well plates | |||
Revision as of 15:44, 20 March 2015
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03/20/2015PCR of g025, g032, and g044 Luc14 and Gal4-EED+dox treated cells with Phusion and P197/198.
Plated cells for transfections tomorrow. Gal4-EED+dox cells plated at 0.16x10^6 cells/well in 12 well plates. Luc14 cells plated at 0.1x10^6 cells / well in 12 well plates. Two plates for each cell type for a total of 4 12-well plates
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