Haynes Lab:Notebook/CRISPR Editing/2015/03/22: Difference between revisions
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Rene M Davis (talk | contribs) |
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Annealing reaction in Surveyor protocol | Annealing reaction in Surveyor protocol | ||
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1ul surveyor enzyme, 1ul enhancer, 60 mins at 42deg | |||
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Dilute 1:10 in ES, run on bioanalyzer | |||
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Revision as of 12:21, 22 March 2015
Project name | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
03/22/2015Column purified unt and g032 treated nested PCR with P149/174. Will set up hybridization of 400ng PCR product in 15ul reaction:
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