Haynes Lab:Notebook/CRISPR Editing/2015/05/02: Difference between revisions
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Rene M Davis (talk | contribs) (Autocreate 2015/05/02 Entry for Haynes_Lab:Notebook/CRISPR_Editing) |
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== | ==05/02/2015== | ||
Gal4-EED cells, +dox for 96 hours, -dox and transfected with either KAH228 or GFP, let grow for about 12 days, transfected with g025, g032, g046, or g048 on 4/29 at 6:30pm in triplicate.<br> | |||
Collected cells in lysis buffer, heat for 18mins at 56deg C, put at -20 | |||
<br><br> | |||
Luciferase assay<br> | |||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''''' | |||
| align="center" style="background:#f0f0f0;"|'''avg''' | |||
| align="center" style="background:#f0f0f0;"|'''std dev''' | |||
|- | |||
| Luc14||2.75||0.08 | |||
|- | |||
| KAH228||1.02||0.06 | |||
|- | |||
| GFP||0.76||0.01 | |||
|} | |||
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|} | |} | ||
__NOTOC__ | __NOTOC__ | ||
Revision as of 20:09, 2 May 2015
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05/02/2015Gal4-EED cells, +dox for 96 hours, -dox and transfected with either KAH228 or GFP, let grow for about 12 days, transfected with g025, g032, g046, or g048 on 4/29 at 6:30pm in triplicate.
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