Haynes Lab:Notebook/CRISPR Editing/2015/05/21: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
Rene M Davis (talk | contribs) |
(fix raw html notebook nav) |
||
(3 intermediate revisions by one other user not shown) | |||
Line 2: | Line 2: | ||
|- | |- | ||
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
|- | |- | ||
| colspan="2"| | | colspan="2"| | ||
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --> | <!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --> | ||
==05/21/2015== | ==05/21/2015== | ||
Picked colonies from KAH228 in MV2 and KAH60 in pCvN transformation plates to miniprep tomorrow<br><br> | |||
Specd plasmid dilution nested PCR products | Specd plasmid dilution nested PCR products | ||
{| {{table}} | {| {{table}} | ||
Line 63: | Line 64: | ||
| Gal4-EED||g034||1||5||2||28.85||13.86||2.00||4.14 | | Gal4-EED||g034||1||5||2||28.85||13.86||2.00||4.14 | ||
|} | |} | ||
1ul surveyor, 1ul enhancer into tubes 1-8, 1ul water, 1ul enhancer into tube 9, 42degC for 1hour<br>2.2ul stop solution, ran on ba | |||
<br><br> | |||
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### --> | <!-- ##### DO NOT edit below this line unless you know what you are doing. ##### --> |
Latest revision as of 00:58, 27 September 2017
Project name | Main project page Previous entry Next entry | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
05/21/2015Picked colonies from KAH228 in MV2 and KAH60 in pCvN transformation plates to miniprep tomorrow
Annealed for surveyor, 20ul annealing reactions because of the lower concentrations
1ul surveyor, 1ul enhancer into tubes 1-8, 1ul water, 1ul enhancer into tube 9, 42degC for 1hour
|