Haynes Lab:Notebook/CRISPR Editing/2015/05/21
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05/21/2015Picked colonies from KAH228 in MV2 and KAH60 in pCvN transformation plates to miniprep tomorrow
Annealed for surveyor, 20ul annealing reactions because of the lower concentrations
1ul surveyor, 1ul enhancer into tubes 1-8, 1ul water, 1ul enhancer into tube 9, 42degC for 1hour |