Haynes Lab:Notebook/CRISPR Editing/2015/05/21: Difference between revisions
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| Gal4-EED||g034||1||5||2||28.85||13.86||2.00||4.14 | | Gal4-EED||g034||1||5||2||28.85||13.86||2.00||4.14 | ||
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1ul surveyor, 1ul enhancer into tubes 1-8, 1ul water, 1ul enhancer into tube 9, 42degC for 1hour | |||
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Revision as of 14:17, 21 May 2015
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05/21/2015Specd plasmid dilution nested PCR products
Annealed for surveyor, 20ul annealing reactions because of the lower concentrations
1ul surveyor, 1ul enhancer into tubes 1-8, 1ul water, 1ul enhancer into tube 9, 42degC for 1hour |