Haynes Lab:Notebook/CRISPR Editing/2015/05/22: Difference between revisions
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{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''Cell type''' | |||
| align="center" style="background:#f0f0f0;"|'''Plasmid 1''' | |||
| align="center" style="background:#f0f0f0;"|'''Plasmid 2''' | |||
| align="center" style="background:#f0f0f0;"|'''Rep''' | |||
| align="center" style="background:#f0f0f0;"|'''260''' | |||
| align="center" style="background:#f0f0f0;"|'''280''' | |||
| align="center" style="background:#f0f0f0;"|'''260/280''' | |||
| align="center" style="background:#f0f0f0;"|'''ng/µL''' | |||
| align="center" style="background:#f0f0f0;"|'''1:10 dil''' | |||
| align="center" style="background:#f0f0f0;"|'''ul dil for 7ng''' | |||
| align="center" style="background:#f0f0f0;"|'''ul water''' | |||
|- | |||
| Luc14||KAH226||g034||1||0.081||0.04||2.021||80.531||4.02655||1.738460965||14.01153903 | |||
|- | |||
| Luc14||KAH226||g034||2||0.126||0.061||2.06||126.451||6.32255||1.107148223||14.64285178 | |||
|- | |||
| Luc14||KAH226||g034||3||0.091||0.044||2.045||90.862||4.5431||1.540798133||14.20920187 | |||
|- | |||
| Luc14||GFP||g034||1||0.104||0.051||2.052||104.239||5.21195||1.343067374||14.40693263 | |||
|- | |||
| Luc14||GFP||g034||2||0.065||0.031||2.094||64.965||3.24825||2.155006542||13.59499346 | |||
|- | |||
| Luc14||GFP||g034||3||0.039||0.02||1.99||39.096||1.9548||3.580928995||12.169071 | |||
|- | |||
| ||||||||||||||||||||15.75 | |||
|} | |||
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Revision as of 14:33, 22 May 2015
Project name | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
05/22/2015SYBR qPCR to determine plasmid counts for dilution experiment
Step 1: make two PCR tubes for each sample with the following volumes of water and gDNA for 2ng of gDNA per well
Step 2: make primer master mixes. Will have to add 3.5x to each triplicate gDNA tube so can calculate mastermixes from that
Step 3: mix gDNA and primer mixes
Step 4: pipette plate
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