Haynes Lab:Notebook/CRISPR Editing/2015/07/28: Difference between revisions
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Line 43: | Line 43: | ||
| Reagent || Vol. μL || (x27) | | Reagent || Vol. μL || (x27) | ||
|- | |- | ||
| 750 nM F/R primer mix || 3.0 || | | 750 nM F/R primer mix || 3.0 || 81.0 | ||
|- | |- | ||
| 2x SYBR (Roche) || 7.5 || | | 2x SYBR (Roche) || 7.5 || 202.5 | ||
|- | |- | ||
| || 10.5 || | | || 10.5 || 283.5 | ||
|} | |} | ||
Template master mixes | Template master mixes | ||
* Multiplier = 3 rxns x 3 reps + 3 | * Multiplier = 3 rxns x 3 reps + 3 = 12 | ||
# CRISPR 1d | # CRISPR 1d | ||
# CRISPR 2d | # CRISPR 2d | ||
Line 62: | Line 62: | ||
# no template control (water) | # no template control (water) | ||
{| {{table}} | |||
|- | |||
| Reagent || Vol. μL || (x12) | |||
|- | |||
| DNA (10 ng) || 3.0 || 81.0 | |||
|- | |||
| 2x SYBR (Roche) || 7.5 || 202.5 | |||
|- | |||
| || 10.5 || 283.5 | |||
|} | |||
Revision as of 13:25, 28 July 2015
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07/28/2015
mCherry qPCR/melt pre-course test (at CSH)
Note: reference primers (GAPDH B2) are not here yet
|