Haynes Lab:Notebook/CRISPR Editing/2015/07/28: Difference between revisions
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** P145/146 (...@255) = 100 bp; Tm = 57, 58.8°C | ** P145/146 (...@255) = 100 bp; Tm = 57, 58.8°C | ||
* Number of unique reactions: 8 x 3 = 24 reactions | * Number of unique reactions: 8 x 3 = 24 reactions | ||
* Number of wells needed: 24 x 3 = '''72 wells''' (1 plate) | * Number of wells needed: 24 rxns x 3 reps = '''72 wells''' (1 plate) | ||
Primer master mixes | Primer master mixes | ||
* Multiplier = 8 x 3 + 3 = 27 | * Multiplier = 8 rxns x 3 reps + 3 = 27 | ||
# P147/148 | # P147/148 | ||
# P143/144 | # P143/144 | ||
# P145/146 | # P145/146 | ||
Note: reference primers (GAPDH B2) are not here yet | |||
{| {{table}} | {| {{table}} | ||
|- | |- | ||
| Reagent || Vol. || (x27) | | Reagent || Vol. μL || (x27) | ||
|- | |- | ||
| 750 nM F/R primer mix || | | 750 nM F/R primer mix || 3.0 || ## | ||
|- | |- | ||
| 2x SYBR (Roche) || | | 2x SYBR (Roche) || 7.5 || ## | ||
|- | |||
| || 10.5 || ## || | |||
|} | |||
Template master mixes | Template master mixes | ||
* Multiplier = 3 rxns x 3 reps + 3 | |||
# CRISPR 1d | |||
# CRISPR 2d | |||
# CRISPR 3d | |||
# CRISPR 4d | |||
# donor Stop@72 | |||
# donor Stop@162 | |||
# donor Stop@255 | |||
# no template control (water) | |||
Revision as of 13:19, 28 July 2015
Project name | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | |||||||||||||
07/28/2015
mCherry qPCR/melt pre-course test (at CSH)
Note: reference primers (GAPDH B2) are not here yet
|