Haynes Lab:Notebook/CRISPR Editing/2015/08/17: Difference between revisions

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{| {{table}}
| align="center" style="background:#f0f0f0;"|'''gRNA'''
| align="center" style="background:#f0f0f0;"|'''F primer'''
| align="center" style="background:#f0f0f0;"|'''R primer'''
|-
| 23||P159||P176
|-
| ||||
|-
| 30||P159||P215
|-
| ||||
|-
| 29||P149||P174
|-
| 35||P149||P174
|-
| 36||P149||P174
|-
| ||||
|-
| 33||P149||P176
|-
| ||||
|-
| 38||P165||P160
|-
| ||||
|-
| 41||P175||P214
|-
| ||||
|-
| 42||P213||P214
|-
| 43||P213||P214
|-
| 44||P213||P214
|-
| 49||P213||P214
|-
| ||||
|-
| 40||P163||P160
|-
| 45||P163||P160
|}
gRNA F primer R primer
23 P159 P176
30 P159 P215
29 P149 P174
35 P149 P174
36 P149 P174
33 P149 P176
38 P165 P160
41 P175 P214
42 P213 P214
43 P213 P214
44 P213 P214
49 P213 P214
40 P163 P160
45 P163 P160
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->
|}
|}


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Revision as of 09:36, 17 August 2015

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08/17/2015

Phusion PCR for singlet full coverage of all gRNAs tested

gRNA F primer R primer
23 P159 P176
29 P149 P174
30 P159 P215
33 P149 P176
35 P149 P174
36 P149 P174
38 P165 P160
40 P163 P160
41 P175 P214
42 P213 P214
43 P213 P214
44 P213 P214
45 P163 P160
49 P213 P214


gRNA F primer R primer
23 P159 P176
30 P159 P215
29 P149 P174
35 P149 P174
36 P149 P174
33 P149 P176
38 P165 P160
41 P175 P214
42 P213 P214
43 P213 P214
44 P213 P214
49 P213 P214
40 P163 P160
45 P163 P160


gRNA F primer R primer 23 P159 P176

30 P159 P215

29 P149 P174 35 P149 P174 36 P149 P174

33 P149 P176

38 P165 P160

41 P175 P214

42 P213 P214 43 P213 P214 44 P213 P214 49 P213 P214

40 P163 P160 45 P163 P160


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